Interstate 71 near the Oldham-Jefferson line is covered in blue and yellow ink and northbound lanes will be closed until late afternoon.
Oldham County Police spokesperson Billy Way said a tractor-trailer crashed about 10:20 a.m., spewing 500 containers of blue and yellow ink across the interstate.
No injuries have been reported.
The truck overturned between the Oldham-Jefferson line near the rest area, and came to rest in several pieces, Way said.
Emergency crews have determined that the ink is a non-hazardous material.